Expression signatures of cells with circular chromosomes. For each condition, the 51 genes from Table 2 that had expression changes of twofold or more in both strains C1 and C5, but not in survivors with linear chromosomes, are graphed in clusters of vertical bars. The height of each bar represents fold-change in expression relative to wild type. Survivors with linear or circular chromosomes are labeled. Strains H1 and H2 have circular chromosomes as evidenced by their inability to enter into a pulsed-field gel (data not shown). Strains H1 and H2 were not used to derive the expression signature and are shown as an independent verification of it.