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Table 4 Toxicity of fragments versus whole ORF products

From: Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

ORF/Gene name Gene description Phenotype of gene deletion Conserved domain or TMS in entire protein Phenotype of gene overexpression Conserved domain or TMS in insert Phenotype of insert overexpression
YDL112w/TRM3* tRNA 2'-O-ribose methyltransferase Viable SpoU_methylase 3/3 - 3/1
YML128C/MSC1/ GIN3* Weak similarity to Schizosaccharomyces pombe stress protein Viable 1 TMS 3/3 - 3/0
YGR149w/_* ठSimilar to S. pombe hypothetical protein Viable 5 TMS 3/2 to 3/3 3 TMS 3/2
YGL023c/PIB2* § Phosphatidylinositol 3-phosphate binding Viable FYVE 3/1 FYVE 3/0
YPL043w/NOP4 Nucleolar protein, RNA processing Lethal RRM (4 motifs) 3/0 Bias D, E, K 3/0
YOR166c/_ * § Similarity to hypothetical S. pombe protein Viable PINc (nucleotide binding) 3/0 PINc 3/0
YJL212c/OPT1¶¥ Oligopeptide transporter Viable OPT 3/1 2 TMS, OPT 3/1
YNL003c/PET8¥¤ Mitochondrial carrier Viable mito_carrier 3/2 mito_carrier 3/2
YJL092w/HPR5¥# DNA helicase involved in DNA repair Viable UvrD 2/0 UvrD (central) 3/2
YMR190c/SGS1¥¤ DNA helicase of DEAD/DEAH family Viable DEAD, HELICc, HRDC 3/0 DEAD 3/2
YNL033W/_§ Strong similarity to YNL019c Viable 2 TMS 3/1 1 TMS 3/2
YHR067w/_* § Weak similarity to S. pombe hypothetical protein Viable Maoc : Acyldehydratase 3/1 MaoC 3/2
YGL263w/COS12§¥¤ Similarity to subtelomeric encoded proteins Viable DUP 3/0 DUP 3/1
  1. Systematic nomenclature and gene name, where applicable, are given in the first column. *Singleton: the gene has no paralog in S. cerevisiae. Gene fragment and #entire gene, respectively, were already known as toxic upon overexpression. Putative uncharacterized transporter (see [35]). §Gene of unknown classification. Two non-overlapping inserts of the ORF were selected. ¥One or several paralogs of this gene have also been selected as toxic inserts in this work (see Additional data file 3). ¤Gene having a paralog in S. cerevisiae already known as toxic upon overexpression. Columns 2 and 3 contain respectively a brief description of the function of the gene product and the phenotype of the disruption mutant (MIPS [38]). The results of a search for conserved domains is shown in columns 4 (in whole protein) and 6 (in inserts). Phenotypes in uninduced and overexpression conditions of the entire gene and of fragments are given in columns 5 and 7 respectively (see Figure 3 for illustrations of the phenotypes).