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Table 1 Distribution of the toxic inserts between the different genetic objects

From: Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae

Genetic objects represented Number of toxic inserts Percentage of total Mean size ± SD (nucleotides) (minimum-maximum) Phenotypes Inserts encoding artificial peptides
     3/0, 3/1 3/2 2/0, 2/1 1/0  
In-frame ORF fragments 493 68.7 743 ± 311 (220-2,120) 375 87 23 8 _
Antiparallel ORF fragments 68 9.6 532 ± 247 (140-1,220) 37 11 12 8 53
Out-of-frame ORF fragments 53 7.5 733 ± 306 (170-1,620) 12 11 22 8 12
Intergenic regions 41 6.0 625 ± 358 (170-1,820) 13 4 16 8 27
LTRs 2 0.3 595 (320-1,120) 1 0 0 1 1
Ty elements 15 (10) 2.1 633 ± 265 (320-870) 7 4 2 2 _
Y' elements 9 (3) 1.2 678 ± 370 (320-1,320) 9 0 0 0 6
RNA genes 4 0.5 662 ± 246 (470-1,020) 3 0 1 0 3
2 μm plasmid 17 (10) 2.4 564 ± 288 (170-1,220) 13 3 1 0 5
Mitochondrial DNA 12 1.7 483 ± 201 (200-920) 9 3 0 0 10
Total 714 100 703 ± 313 (140-2,120) 479 123 77 35 117
  1. The first column indicates nature of sequence in toxic inserts. Second and third columns contain, respectively, actual number of inserts of each type and corresponding percentages. For Tys, Y' and 2 μm plasmid, numbers in brackets represent numbers of in-frame fragments of natural ORFs. The fourth column shows the mean size of insert in nucleotides ± standard deviation (SD) with minimum and maximum sizes in brackets. Scoring of each type of phenotype is shown in the next four columns. The last column shows the number of inserts in which artificial ORFs of more than 24 codons were detected.