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Table 1 Protein preparation and characterization

From: A catalog of human cDNA expression clones and its application to structural genomics

hEx1 clone*

pQTEV subclone

GenBank accession number

GenBank protein name

Protein yield per culture volume (mg/l)

Free of aggregation§ (DLS)

Melting temperature (°C)

P08596

758H0126

AAH01214

CGI-68

1.5

Yes

60.3 (1)

H05552

758H0426

AAF76210

DRG-1

1.5

No

54.8 ± 0.8 (2)

E10541

758G1126

AAH07250

Coatomer protein complex, subunit ε

1.7

No

50.8 (1)

E12601

758H1026

CAC37950

HCC-1

7

No

No peak

E09507

758C041

AAB59004

ARA9

4

Yes

54.7 ± 0.4 (2)

I07544

250H071

AAH05289

Thioredoxin-like

7

Yes

56.6 ± 2.9 (3)

N13557

250B082

AAH06346

Aortic preferentially expressed p. 1

18

Yes

55.5 ± 2.2 (4)

J17573

250A012

AAA58682

Transformation-sensitive protein

19

Yes

51.1 (1)

G03570

250A062

AAH11960

Gankyrin

3

Yes

No data

L02591

250B092

AAH12995

Protein for MGC:3363

8

No

41.4 (1)

C16543

250B062

AAH04430

Unknown

2

Yes

49.2 (1)

H07543

250B112

BAA78534

Adenylate kinase 1

38

Yes

51.6 (1)

H14508

250F062

BAA04802

HUM22SM

38

No data

53.4 (1)

E07518

250A053

AAF87955

NBP

7

Yes

32.0 (1)

H08589

250A073

AAH07873

Unknown

3

No

No peak

A23602

250C112

CAB53072

Microtubule-associated protein

4

No

58.0 (1)

M05547

250H122

AAH15027

Prolidase

10

No

50.8 ± 1.3 (3)

  1. *RZPD clone identifiers without prefix 'MPMGp800'. Clone in vector pQTEV used for protein production, RZPD clone identifiers without prefix 'PSFEp'. Protein yield of one typical protein preparation. §As determined from measurements of purified protein preparations of 0.2-1 mg/ml. Melting temperature determined by DSC. The number of independent measurements is indicated in brackets. Proteins for which no peak was observed were considered to be unfolded.