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Table 1 Protein preparation and characterization

From: A catalog of human cDNA expression clones and its application to structural genomics

hEx1 clone* pQTEV subclone GenBank accession number GenBank protein name Protein yield per culture volume (mg/l) Free of aggregation§ (DLS) Melting temperature (°C)
P08596 758H0126 AAH01214 CGI-68 1.5 Yes 60.3 (1)
H05552 758H0426 AAF76210 DRG-1 1.5 No 54.8 ± 0.8 (2)
E10541 758G1126 AAH07250 Coatomer protein complex, subunit ε 1.7 No 50.8 (1)
E12601 758H1026 CAC37950 HCC-1 7 No No peak
E09507 758C041 AAB59004 ARA9 4 Yes 54.7 ± 0.4 (2)
I07544 250H071 AAH05289 Thioredoxin-like 7 Yes 56.6 ± 2.9 (3)
N13557 250B082 AAH06346 Aortic preferentially expressed p. 1 18 Yes 55.5 ± 2.2 (4)
J17573 250A012 AAA58682 Transformation-sensitive protein 19 Yes 51.1 (1)
G03570 250A062 AAH11960 Gankyrin 3 Yes No data
L02591 250B092 AAH12995 Protein for MGC:3363 8 No 41.4 (1)
C16543 250B062 AAH04430 Unknown 2 Yes 49.2 (1)
H07543 250B112 BAA78534 Adenylate kinase 1 38 Yes 51.6 (1)
H14508 250F062 BAA04802 HUM22SM 38 No data 53.4 (1)
E07518 250A053 AAF87955 NBP 7 Yes 32.0 (1)
H08589 250A073 AAH07873 Unknown 3 No No peak
A23602 250C112 CAB53072 Microtubule-associated protein 4 No 58.0 (1)
M05547 250H122 AAH15027 Prolidase 10 No 50.8 ± 1.3 (3)
  1. *RZPD clone identifiers without prefix 'MPMGp800'. Clone in vector pQTEV used for protein production, RZPD clone identifiers without prefix 'PSFEp'. Protein yield of one typical protein preparation. §As determined from measurements of purified protein preparations of 0.2-1 mg/ml. Melting temperature determined by DSC. The number of independent measurements is indicated in brackets. Proteins for which no peak was observed were considered to be unfolded.