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Table 3 Validation of array data by QRT-PCR

From: Function-informed transcriptome analysis of Drosophila renal tubule

Gene MAS enrichment SAM enrichment QRT-PCR enrichment
Highly enriched    
CG13665 98.9 8.7 9.0
CG14957 95.9 21.9 23.8
CG13905 22.6 17.4 110
CG13836 80.6 30.1 11.7
Evenly expressed    
CG17737 1.0 0.9 0.74
CG10731 1.0 1.1 0.68
CG8327 1.0 0.8 1.2
Arp66 1.0 1.1 0.47
Highly depleted    
CG13421 0.00 0.067 0.19
CG12408 0.01 0.11 0.14
Act88F 0.01 0.14 0.03
CG15575 0.01 0.082 0.008
  1. Enrichment in tubule mRNA compared to whole fly mRNA, computed from the microarray dataset with MAS 5.0 or SAM (see text), were compared with real values obtained by QRT-PCR. Four separate fly and tubule samples were run with primers for each gene, and for rp49, a ribosomal gene generally considered to be invariant. RNA quantities were calculated, and the gene:rp49 ratio calculated for each sample pair. Tubule enrichment was calculated as the (gene:rp49)tubule/(gene:rp49)fly.