Correlation between H3 and FLAG-H2B ChIP datasets. DNA associated with histones in vivo was enriched in ChIP assays using antibodies against histone H3 or FLAG-H2B, and quantified by microarrays. (a) Relative enrichment of promoters and other non-coding regions in the H3 and H2B ChIP assays is shown. (b) Histogram showing distributions of enrichment for promoter regions in the H3 and H2B ChIP assays. (c) Overlap between regions depleted in the H3 and FLAG-H2B assays is shown. Overall, there is an 0.83 correlation between these ChIP datasets. (d) Overlap between regions depleted in the H3 ChIP assay and regions enriched by aqueous extraction is shown .