Table 1 Htr1-Htr18 chemotaxis annotations
Gene name | Name | Sensing domain | Length | HAMP domain | Membrane regions | Method | Role/responds to | Annotation |
|---|---|---|---|---|---|---|---|---|
htr1 | VNG1659g | sop1 | 536 | 35-104 | 12-31 | Known | Light | Responds to light via sensory rhodopsin |
htr2 | VNG1765g | sop2 | 764 | 283-352 | 13-35 | Known | Light | Responds to light via sensory rhodopsin |
htr3 | VNG1856g | self/? | 633 | 125-195 | 125-144 | Known | Amino acids | Â |
htr4 | VNG0806g | self/? | 778 | 298-367 | 29-48, 297-319 | - | - | Â |
htr5 | VNG1760g | ProX | 810 | 325-394 | 35-57, 325-344 | Known | Amino acids, osmoprotectants | ProX is a putative glycine betatine/choline/proline substrate-binding protein |
htr6 | VNG0793g | yufN | 545 | 295-365 | 21-43, 297-319 | 3D-Jury | Sugars | yufN is annotated as an ABC transporter and lipoprotein META-SERVER/3D-Jury finds a strong hit to 2 gbp D-galactose/D-glucose periplasmic binding protein |
htr7 | VNG1759g | VNG1758H | 789 | - | 1-91 | Rosetta | - | Weak hit to sensory rhodopsin |
htr8 | VNG1523g | self | 633 | - | 48-206 | Known | Oxygen | Experimentally known to play a role in aerotaxis |
htr9 | VNG1395g | self/? | 481 | - | - | Pfam | Redox/o2/light | PF0989, PAS domain |
htr10 | VNG1505g | self/? | 489 | - | - | Rosetta PSIBLAST | Oxygen | Domain 1 rosetta hit to 1ljwA hemoglobin Domain 1 hit to 1or4A via PSI-BLAST (recent PDB) |
htr12 | VNG1442g | self/ VNG1440H | 420 | - | - | Rosetta 3D-jury Pfam | Redox/o2/light | htr12 has amino-terminal (domain 1) hit to PF0989 (PAS domain) htr12-domain 1 also has a 3d-Jury hit to 1dp6A (FixL heme domain) VNG1440H has a weak rosetta hit to 2occC2 (cytochrome C subunit) and predicted TM helices |
htr13 | VNG1013g | self/? | 423 | - | - | Rosetta | Peptides/? | Hit to 1jwfA (Gga1, involved in protein transport) Htr13 could be involved in response to peptides in the environment |
htr14 | VNG0355g | self/? | 627 | 58-129 | 36-58 | Pfam | Peptides/? | Weak hits to PF01920-KE2 domain, PF02996-prefoldin |
htr15 | VNG0958g | VNG0959H | 636 | - | - | 3D-jury | Oxygen | Domain 2 has 3d-jury hit to 1dp6A (FixL Heme domain) |
htr16 | VNG0614g | self/ VNG0613H | 628 | 129-199 | 130-152 | Pfam | Lipids/? | Hit to PF01442 in domain 2 of htr16 (PF01442 is an apolipoprotein Involved in the uptake of lipids and/or cholesterol) |
htr17 | VNG1733g | VNG1734H | 536 | - | 1-91 | - | - | Not applicable |
htr18 | VNG0812g | PotD | 790 | 257-327 | - | known | Lipids | PotD is a spermidine/putrescine binding protein |