Tissue-specific radial positioning of chromosomes. (a) FISH analysis of chromosome 5 (green) in liver, lymphocytes and lung cell nuclei. DNA counterstaining with DAPI is in blue. Chromosome 5 is preferentially enriched in the nuclear interior in liver, within the medial nuclear subvolume in lymphocytes, and at the nuclear periphery in lung cells. Scale bar, 2 μm. (b) Quantitation of the radial distribution of chromosomes in different cell types. Data was binned in five concentric shells of equal volume designated 1 to 5 from the center of the nucleus to the periphery. (c) Pairwise comparison of chromosome radial distribution using contingency table analysis. p-values < 0.05 (yellow/red) were considered significant. Ki, kidney; Li, liver; LL, large lung cells; Ly, lymphoblasts; My, myeloblasts; SL, small lung cells. Between 41 and 180 cells were analyzed per tissue.