Figure 5

Meta-analysis of Arbeitman et al. [20, 36] data. (a,b) Scatterplots of averaged (Ln) ratios for (a) male versus female and (b) 'tud' male versus 'tud' female comparing data from experiments performed with the FlyGEM and GPL218 platforms. Two-fold sex-biased expression in both datasets are indicated in blue (male-biased) and pink (female-biased). Those showing two-fold male-biased expression in one data set, but two-fold female-biased expression in the other are shown in yellow. (c,d) Comparison of Northern expression ratios for 16 probes versus averaged (Ln) ratios from Arbeitman et al. are shown in scatterplots for (c) male versus female and (d) 'tud' male versus 'tud' female. The 16 probes represent CG13263, CG8994, CG3972, CG10701, CG1088, CG7961, G10961, CG6206, CG4586, CG13095, CG5089, CG4847, CG6483, CG8549, CG7660, CG6457. Two-, four- and 10-fold deviations from 1:1 ratio are indicated by yellow, red and blue color (see Figure 4). (e,f) Clustering of FlyGEM normalized intensity data was performed for sex-biased somatic genes from Table 2 of Arbeitman et al. [36]. (e) 33 male and (f) 26 female genes are shown as intensity heat clustergrams. The normalized intensities are indicated as high expression (yellow), moderate (red), low (blue) and below background (black). (Intensities are represented by color as in Figure 4). Columns show 52 channels parsed from 26 pairwise FlyGEM microarray experiments. Clustering shows soma-bias corroborating the Table 2 lists. The array element species represented in rows from top are listed as follows: (e) CG12268, CG3359, CG3359, CG3359, CG5740, CG7050, CG7157, CG7178, CG7178, CG7748, CG8110, CG8552, CG9456, CG12558, CG14024, CG15097, CG16820, CG17843, CG18284, CG3359, CG5411, CG5455, CG6518, CG6716, CG6844, CG7178, CG7178, CG7342, CG8708, CG8708, CG8909, CG9519; (f) CG10281, CG10566, CG1090, CG11248, CG12269, CG1646, CG17012, CG7129, CG7702, CG7899, CG8327, CG8370, CG9547, CG10944, CG12740, CG14792, CG18525, CG3195, CG3751, CG4087, CG5821, CG7777, CG8453, CG8705, CG9696, CG9699.