Microarray experimental design. Sex biased gene expression data was derived from 44 microarray hybridizations testing 15 conditions. All experimental conditions included at least one biological replicate and most also include dye-flip hybridizations for additional replicates. Abbreviated genotypes of the samples are shown in light ellipses with the total number of replicated hybridizations in dark circles between the two samples. Full genotypes of the flies used are as follows: X/Y;tra2- males are w67c/BsY;tra2B/Df(2R)trix, X/X;tra2- sex transformed males are w67c/+;tra2B/Df(2R)trix, X/X;dsxD/dsx- males are (+/+; dsxM+R45/dsxswe. 'tud' males and females are the progeny of homozygous tud1bw1sp1females mated to tud1bw1sp1/CyO males and themselves are genotypically tud1bw1sp1homozygotes. Females and males are whole adult y1w67cflies. Ovary, testis and no gonad samples are also derived from y1w67cflies.