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Table 1 Analysis of transcripts represented in conventional and oligo-capped cDNA libraries

From: Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensisand the evolution of secreted proteins in parasites

 

Conventional cDNA libraries

Oligo-capped cDNA library

Total sequences providing peptide predictions

734

500

In-frame ATG followed by ≥ 99-nucleotide open reading frame (ORF)

567 (77.2%)

430 (86.0%)

Predicted ORF length (average)

114.6

101.5

% Signal peptide or signal anchor

SP: 74 (10.1%)

SP: 102 (20.4%)

 

SA: 16 (2.2%)

SA: 5 (1.0%)

% Spliced leader

0

37 (7.4%)