Figure 2From: Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensisand the evolution of secreted proteins in parasitesProportion of ESTs predicted to encode signal sequences. (a) EST sequences were classified as conserved (similarities to non-nematode database entries), nematode-specific (similarities only to C. elegans or other nematode sequences), or novel (no similarities to existing entries), using a cutoff score of 80 in BLASTX (P < e-10). The number of ESTs bearing potential signal sequences was then calculated and the results are shown here. (b) Effects of relaxing cutoff scores on distribution of signal peptide-containing predicted gene products among conserved, nematode-specific and novel categories. Numbers of clusters in each category are given for cutoffs of 80 (P <e-10), as used in (a), and 50 (P <e-6).Back to article page