Figure 1

Multiple alignment of the KAP family NTPases. The secondary structure predicted by the PHD program is displayed above the alignment, where E designates a β-strand and H designates α-helix. The helix and strand numbering is given for the secondary structural elements of the conserved P-loop fold. The 80% consensus coloring reflects the following amino acid classes: h (hydrophobic residues: ACFILMVWY), a (aromatic residues: FHWY), and l (aliphatic residues: VIL) are shaded yellow; b (big residues: LIYERFQKMW) are shaded gray; p (polar residues: CDEHKNQRST), - (acidic residues: DE), + (basic residues: HKR) and c (charged residues:HRKDE) are colored magenta; o (alcohol-group-containing residues: ST) are colored blue; s (small: GASCVDNPT) and u (tiny: GAS) residues are colored green. The protein identifiers in the alignment include the name of the protein/gene, species abbreviation and the GenBank gi separated by underscores. The groups discussed in the text are indicated to the right in the last block of the alignment. The asterisk next to the rat sequence indicates a Kidins paralog with a potentially inactive NTPase domain. Species abbreviations are as follows: Atu: Agrobacterium tumefaciens, Ana: Anabaena sp pcc 7120, Ce: Caenorhabditis elegans, Cpe: Clostridium perfringens, Cgl: Corynebacterium glutamicum, Ceff: Corynebacterium efficiens, Dr: Deinococcus radiodurans, Dm: Drosophila melanogaster, Ec: Escherichia coli, Plaf: F plasmid, Gsu: Geobacter sulfurreducens, Hs: Homo sapiens, Kpne: Klebsiella pneumoniae, Lme: Leuconostoc mesenteroides, Mcsp: Magnetococcus sp mc-1, Mde: Microbulbifer degradans, Npu: Nostoc punctiforme, Pput: Pseudomonas putida, Pfl: Pseudomonas fluorescens, Psy: Pseudomonas syringae, Rme: Ralstonia metallidurans, Rn: Rattus norvegicus, Step: Staphylococcus epidermidis, Ssp: Synechocystis sp, Tm: Thermotoga maritima, Vpar: Vibrio parahaemolyticus, Vvul: Vibrio vulnificus.