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Table 1 Antibodies used in Figure 5 and 5a summary of inter-set correlations

From: Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements

 

Antibody name

RCA/direct (Figure 5a)

RCA/indirect (Figure 5b)

RCA/indirect (Figure 5c)

AB01

VAI00101 anti-cathepsin B (ab-1)

-/-

0.99/-

0.83*/-

AB02

VAI00194 anti-von Willebrand factor

0.94/0.99

0.94/0.8

0.79/0.6

AB03

VAI00198 anti-uPA

-/-

-/-

0.95/0.95

AB05

VAI00233 anti-LD1234

0.8/-

0.8/-

0.95/-

AB06

VAI00243 anti-IL-1alpha

0.99/-

0.99/-

0.97/0.98

AB07

VAI00244 anti-IGG1

0.94/0.95

0.94/0.67

0.87/0.74

AB08

VAI00245 anti-complement C3

0.82*/0.97

0.82*/-

0.99/0.68*

AB09

VAI00246 anti-complement C4

0.93/0.98

0.93/0.79

0.78/0.81

AB10

VAI00261 anti-VEGF

0.84*/0.95

0.84*/-

0.77/0.69

AB11

VAI00263 anti-alpha2-macroglobulin

0.71/0.99

0.71/0.77

0.67/0.91

AB12

VAI00269 anti-IL-6

0.74*/-

0.74*/-

0.7/-

AB13

VAI00273 anti-ceruloplasmin

-/0.71

-/-

0.98/0.87

AB14

VAI00274 anti-AP

0.98/0.93

0.98/0.74

0.9/0.77

AB15

VAI00276 anti-Alpha-1-AT

0.95/0.95

0.95/-

0.75/0.86

AB16

VAI00277.1 anti-haptoglobulin

0.96/-

0.96/-

0.95/0.89

AB17

VAI00282 anti-alpha-fetoprotein

0.98/0.98

0.98/0.98

0.89/0.9

AB18

VAI00297 anti-Timp-1

-/-

-/-

0.96/0.96

AB19

VAI00298 anti-IGFBP-3

0.99/0.97

0.99/0.69

0.86/0.26*

AB20

VAI00300 anti-IL-8

0.99/0.96

0.99/0.77

0.92/0.97

AB21

VAI00303 anti-VEGF

0.63*/0.83

0.63*/0.77*

0.66/0.87

AB22

VAI00305 anti-IL-6

-/-

-/-

0.93/0.95

AB23

VAI00307 anti-IL-2

0.78/0.96

0.78/-

0.87/0.63

AB24

VAI00308 anti-TSP-1

0.91/0.95

0.91/-

0.9/-

AB25

VAI00338 anti-plasminogen

0.96/-

0.96/-

0.88/0.89

AB26

VAI00339 anti-CA125

0.92*/-

0.92*/-

0.94/0.76

AB27

VAI00342 anti-CEA

0.93/0.91

0.93/0.73

0.84/0.71

AB28

VAI00348 anti-beta2-microglobulin

0.98/-

0.98/-

0.88/0.91

AB29

VAI00352 anti-PAI

0.77/0.87

0.77/-

0.95/0.87

AB30

VAI01032 anti-alpha1 ACT

0.96/0.92

0.96/0.95

0.91/0.95

AB31

VAI01126 anti-albumin

0.88/0.94

0.88/0.72

0.82/0.56*

AB32

VAI10003 anti-IgG-Fc

0.98/0.98

0.98/0.95

0.87/0.95

AB33

VAI10007 anti-hemoglobin

0.97/0.99

0.97/1

0.87/0.8

AB34

VAI10011 anti-IgA

0.92/0.99

0.92/0.86

0.88/0.97

AB35

VAI10013 anti-transferrin

0.82/0.96

0.82/0.75

0.63/0.79

  1. For each antibody, the correlation between replicate sets of 24 microarrays is given, both for RCA and for direct labeling or indirect detection. The correlation is the Pearson correlation in the overlap between the sets, that is, using only samples for which measurements were available in both sets. Correlations were not calculated if the overlap between the duplicate sample sets was less than three samples or less than half the samples from one of the sets. Asterisk, not a statistically significant correlation, using a 99% confidence level based on the size of overlap between sets.
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Genome Biology

ISSN: 1474-760X

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