Refinement of Oriscan predictions. The number of matching (green) and total (black) predictions at different stages in the algorithm are shown. From the 12 million positions in the yeast genome, the best 11,800 matches to the core ACS were selected, and these matched 354 members of the ORC/MCM evaluation set (ACS). Selection against poly-T sequences removed 5,268 predictions, leaving 6,532, including 332 matches to the ORC/MCM set (non-T). Further selection using the 268 nucleotide matrix containing flanking sequence removed 4,632 predictions, leaving 1,900, including 257 matches (flanking). These predictions were then ranked; the top 350 contained 179 matches, and the top 100 contained 84 matches.