MYRbase: analysis of genome-wide glycine myristoylation enlarges the functional spectrum of eukaryotic myristoylated proteins

Table 1 Numbers of analyzed sequences, experimentally verified myristoylated proteins plus their homologs and the set of additional new predictions

	Number in GenBank*	Number experimentally verified + homologs^†	Number established NEW true predictions^‡
Total	600,916	1,122	2,037
Leading methionine^§	426,128	997	1,548

*Eukarya, non-identical sequences (nr100), February 2003. ^†Experimentally verified myristoylated proteins and their homologs with conserved myristoylation site (BLASTP E-value <0.005, plus manual curation). ^‡Estimated NEW true predictions: (total number of predictions) minus (expected number of false-positive predictions) minus (number of experimentally verified + homologs) = number of true predictions not closely related to already experimentally verified examples (possibly additional new functions for myristoylated proteins?). ^§When a leading methionine is present in the sequence, it is less likely to represent a non-amino-terminal fragment in the database. However, this also excludes some true amino-terminal fragments, where a glycine becomes amino-terminal after proteolytic cleavage.

ISSN: 1474-760X