Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues

Table 1 Confirmation of microarray data by northern blot and real-time RT-PCR assays

Description hit	GenBank accession number	Method used	Adult leaf	Seedling leaf	14-day-old root	Immature ear	Emerging tassel
RuBisCO small subunit	AI855224	Microarrays	-1.22	0.20	0.33	0.68	0.61
		Northern blot	-1.30	0.30	ND	ND	ND
Ribosomal protein L11, cytosolic	AI948309	Microarrays	1.09	1.05	0.28	0.54	1.45
		Northern blot	1.50	1.30	0.40	0.30	1.70
Cinnamyl alcohol dehydrogenase	AW927923	Microarrays	1.19	1.22	-0.63	F	0.40
		Northern blot	2.50	1.50	ND	ND	-0.20
Histone deacetylase	AW438666	Microarrays	1.07	-0.69	1.04	0.84	0.69
		Real-time RT-PCR	1.49	-0.29	1.09	ND	0.86
Cysteine proteinase	AW129800	Microarrays	1.08	0.19	0.09	F	0.51
		Real-time RT-PCR	3.19	0.89	0.82	ND	0.75
Methyl-binding protein	AW737448	Microarrays	F	1.31	1.03	-0.30	-0.11
		Real-time RT-PCR	0.79	1.11	1.07	ND	0.24
Cytosine 5' DNA methyltransferase	AW215926	Microarrays	1.75	0.02	0.79	-0.32	0.56
		Real-time RT-PCR	2.27	-0.88	-0.51	ND	0.46
Membrane protein Mlo5	BE025314	Microarrays	F	1.04	1.20	-0.25	-0.10
		Real-time RT-PCR	0.97	1.62	1.34	ND	0.42
snRNP Sm protein F	AW330881	Microarrays	2.72	1.84	1.02	-1.06	0.39
		Real-time RT-PCR	1.69	5.25	1.92	ND	0.71
	AW433427	Microarrays	3.04	1.03	-1.29	-0.99	0.21
		Real-time RT-PCR	2.12	1.21	-1.07	ND	0.79

The numbers correspond to the log₂ ratios. The transcripts that are upregulated by UV-B by more than two-fold are in bold type, while transcripts downregulated by UV-B by more than two-fold are in italic. F, flagged ESTs which had signals similar to the background in some condition and were eliminated during the analysis; ND, not determined.

ISSN: 1474-760X