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Table 1 Confirmation of microarray data by northern blot and real-time RT-PCR assays

From: Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues

Description hit GenBank accession number Method used Adult leaf Seedling leaf 14-day-old root Immature ear Emerging tassel
RuBisCO small subunit AI855224 Microarrays -1.22 0.20 0.33 0.68 0.61
   Northern blot -1.30 0.30 ND ND ND
Ribosomal protein L11, cytosolic AI948309 Microarrays 1.09 1.05 0.28 0.54 1.45
   Northern blot 1.50 1.30 0.40 0.30 1.70
Cinnamyl alcohol dehydrogenase AW927923 Microarrays 1.19 1.22 -0.63 F 0.40
   Northern blot 2.50 1.50 ND ND -0.20
Histone deacetylase AW438666 Microarrays 1.07 -0.69 1.04 0.84 0.69
   Real-time RT-PCR 1.49 -0.29 1.09 ND 0.86
Cysteine proteinase AW129800 Microarrays 1.08 0.19 0.09 F 0.51
   Real-time RT-PCR 3.19 0.89 0.82 ND 0.75
Methyl-binding protein AW737448 Microarrays F 1.31 1.03 -0.30 -0.11
   Real-time RT-PCR 0.79 1.11 1.07 ND 0.24
Cytosine 5' DNA methyltransferase AW215926 Microarrays 1.75 0.02 0.79 -0.32 0.56
   Real-time RT-PCR 2.27 -0.88 -0.51 ND 0.46
Membrane protein Mlo5 BE025314 Microarrays F 1.04 1.20 -0.25 -0.10
   Real-time RT-PCR 0.97 1.62 1.34 ND 0.42
snRNP Sm protein F AW330881 Microarrays 2.72 1.84 1.02 -1.06 0.39
   Real-time RT-PCR 1.69 5.25 1.92 ND 0.71
  AW433427 Microarrays 3.04 1.03 -1.29 -0.99 0.21
   Real-time RT-PCR 2.12 1.21 -1.07 ND 0.79
  1. The numbers correspond to the log2 ratios. The transcripts that are upregulated by UV-B by more than two-fold are in bold type, while transcripts downregulated by UV-B by more than two-fold are in italic. F, flagged ESTs which had signals similar to the background in some condition and were eliminated during the analysis; ND, not determined.
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