Table 2 Oligonucleotides used for the amplification of the GFP-constructs
Construct | Forward primer | Reverse primer |
|---|---|---|
EGFP-tyrosinase | GAATTCAATGCTCCTGGCTGTTTTGTACTG | GGATCCTTATAAATGGCTCTGATACAAGCTG |
EGFP-lysozyme | GAATTCCATGAGGTCTTTGCTAATCTTGGT | GGATCCGGCAGCTCCTCACAGCCG |
GFP-MRP7 | CGGGATCCAATGTGGAATCCTATTTTACTAGATAC | GGGCATGCTCAAAGCTTGCTCAAAAAAGCCCG |
GFP-GSA | CGGGATCCAATGAGGAAGTCTGAAAATCTTTACCAG | GGGCATGCTCACAACTTCGCAAACACCCGACG |
DsRed2-SKL (COS7 cells) | CGGCTAGCGCTACCGGTCGCCACCATGGCC | CGTCTCGAGTTATAATTTGGACAGGTGGTGGCGGCC |
DsRed-SKL (yeast cells) | AGATCTATGGTGAGGTCTTCCAAG | CTGCAGTTATAATTTGGATAGGATCCCAAGGAACAGATGGTGGCG |