A Drosophila protein-interaction map centered on cell-cycle regulators

Table 3 Overlap of two-hybrid data with datasets enriched for true positives

	Interactions	Overlap with LexA map (N = 1,814)	Overlap with Gal4 map (N = 20,439)	Overlap in common
Yeast interlogs (hub/spoke)*	67,238	23 (1.26%)	251 (1.23%)	4
Yeast interlogs (matrix)*	244,202	25 (1.38%)	294 (1.44%)	4
Worm interlogs*	37,863	3 (0.17%)	61 (0.30%)	0
Drosophila genetic^†	2,751	4 (0.22%)	22 (0.11%)	1
Reference set^‡	47	8 (0.44%)	6 (.03%)	2
Ref set (common BD)^§	20	3 (0.17%)	2 (.01%)	0

*Yeast (S. cerevisiae) and worm (C. elegans) interlogs are predicted interactions between the Drosophila orthologs of interacting yeast and worm proteins; 'hub/spoke' and 'matrix' refer to the methods used to derive predicted binary interactions from the protein complex data (see Materials and methods). ^†Genetic interactions were obtained from Flybase [27]. ^‡The Reference set includes published interactions involving any of the 106 BD proteins in the LexA data. ^§The subset of reference interactions involving proteins successfully used as BDs in both the Gal4 and LexA screens is also shown; no interactions from the reference set were found in both the LexA and Gal4 screens using the same BD baits. The chance of finding the indicated number of overlapping interactions with a random set of interactions was <10^-4 for all but the LexA overlaps with worm interlogs (P < 0.1436) or genetic interactions (P < 0.0024) (Additional data file 6).

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