Cryptosporidium parvum genomic Southern blot. C. parvum genomic DNA, 5 μg per lane. Lanes were probed for the following genes: (1) aminopeptidase N; (2) glucose-6-phosphate isomerase; (3) leucine aminopeptidase; (4) pteridine transporter (BT-1); and (5) glutamine synthetase. Lanes (1-4) were restricted with BamH1 and lane (5) with EcoR1. The ladder is shown in 1 kb increments. See Additional data file 1 for probes and methods.