Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

Table 5 Matching of RescueMu genomic loci to other available databases to determine percentage of genic and repeat loci

Category	Number of genomic loci
Total maize genomic loci discovered	14,265*
Number of genic loci identified by:
Maize EST/cDNA	7,555
Plant EST/cDNA	1,253
Protein database	84 (62%)
GENSCAN prediction	708
Number of genic loci^† (percentage of total)	9,600 (67.3%)
Number of loci matching repeats:
Retrotransposon	1,074
DNA transposon	212
MITEs	193
Centromere-related repeats	57
Telomere-related repeats	3
Unknown repeats	221
Other repeats	8
45S ribosomal DNA (18S + 28S)	23
5S ribosomal DNA	10
Transfer DNA	25
Number of repeat loci^‡ (percentage of total)	1,113 (8%)

*The 14,887 unique loci were collapsed into 14,265 unique loci by linking forward/reverse sequence pairs. This provides a more conservative estimate, but in some cases may have incorrectly combined separate loci. ^†Numbers are cumulative: that is, GSSs were first matched to maize EST/cDNAs, then the unmatched GSSs were screened against other plant EST/cDNAs, and so on. ^‡Numbers are not cumulative: that is, some loci could match to both retrotransposon and DNA transposon sequences.

ISSN: 1474-760X