Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

Table 2 Probable germinal insertions (PGI) based on row and column matches

Grid	Rows (r)	Columns (c)	Transposition frequency (τ)*	Expected PGI (e)^†	Row + column matches (m)	Percentage of expected^‡	Transposition frequency (using row + column)^§
G	46	4	0.68	125.1	149	119%	0.81
H^¶	36	4	0.62	89.3	115	129%	0.80
I	38	5	0.62	117.8	128	109%	0.67
K	30	3	0.66	59.4	32	54%	0.36
M^¶	40	3	1.30	156.0	33	21%	0.28
P	37	4	1.40	207.2	71	34%	0.48
Total				754.8	528	70%

*Expected frequency of PGI was determined from DNA gel blot analysis of frequency of newly transposed RescueMu per plant as stated in Table 1; ^†expected = r × c × τ; ^‡percentage of expected = 100 × m/e; ^§transposition frequency = m/(r × c); ^¶for grids H and M, rows were considered columns and vice versa to simplify calculations.

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