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Table 1 Grid organization and analysis of mutant phenotypes segregating among selfed progeny of grid plants

From: Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

Grid* Year Grid size (row × col) Plasmid rescued Libraries sequenced§ Transposition frequency Independent mutations (% of families)¥
       Seed Seedling
A 1999H 34 × 48 No No 0.07 7.2 4.5
B 1999SD 52 × 48 No No 0.10 8.6 10.1
C 1999B 40 × 40 No No 0.13 8.3 28.3
D 1999S 48 × 48 No No 0.26 8.7 15.1
E 2000H 40 × 48 No No 0.25 8.6 27.0
F 2000AZ 41 × 41 No No 0.57 6.6 19.5
G 2000S 46 × 48 Yes Yes 0.68 5.0 11.9
H 2000B 38 × 36 Yes Yes 0.62 7.5 6.9
I 2000B 38 × 34 Yes Yes 0.62 9.5 9.8
J 2000SD 38 × 45 Yes Survey 0.38 9.8 11.1
K 2001H 30 × 30 Yes Yes 0.66 8.0 20.3
L 2001H 36 × 20 Yes Yes 0.66 12.8 17.4
M 2001AZ 40 × 40 Yes Partial 1.30 8.2 ND
N 2001B 32 × 44 In progress No 0.20 6.3 ND
O 2001S 47 × 48 Yes Survey 0.50 5.2 ND
P 2002H 48 × 48 Yes Yes 1.40 5.9 ND
Q 2002H 48 × 24 Yes Yes 1.00 2.7 ND
R 2002AZ 36 × 36 Yes Survey 0.72 3.7 ND
S 2002SD 48 × 48 Yes Survey 1.00 12.7 ND
T 2002H 48 × 46 Yes Survey 1.00 ND ND
U 2002H 48 × 48 Yes Partial >1.30 ND ND
AA 2002S 48 × 48 Yes Yes 0.60 ND ND
BB 2001B 34 × 48 Yes Survey 0.60 6.2 ND
V 2003AZ 45 × 45 In progress Survey 1.00 ND ND
X 2003SD 44 × 44 In progress Survey 1.00 ND ND
  1. *Grids with a single letter contain mainly plants with a RescueMu pollen parent plus the seed from the ear of the founder male crossed by a non-Mutator line. In grids with a double letter, both parents contained RescueMu. Summer nurseries are designated by year and location: A, Tucson, AZ; B, Berkeley, CA; SD, San Diego, CA; S, Stanford, CA. H indicates the winter Hawaii nursery. Vandalism, animals, and environmental damage in the field resulted in some losses compared to expectation of the ear harvest. Ears with fewer than 100 kernels and those from outcross pollinations of male or female sterile grid plants were not assessed for mutation frequency; these lines are being propagated at the Maize Coop by sib pollination to establish a permanent line for later evaluation and distribution. §Yes, indicates that all rows plus four columns were sequenced with the goal of coverage to a depth such that there was a 80-95% probability that plasmids representing germinal insertions would be identified at least once. Grids listed as partial have limited (40%-80%) depth from some rows. Survey sequencing was performed on several rows and columns on the indicated grids to verify that plasmids organized into library plates contained authentic trRescueMu. Library plates will be available from all grids, including V and X, during 2004 as listed at [31]. Frequency of newly transposed RescueMu per plant based on DNA blot hybridization, sampling 30-200 plants per grid. For grid A only, the data are from plants sibling to those in the grid. ¥Progeny families generated by self-pollination of grid plants were examined for kernel defects before shelling, and seedling traits were scored on up to 30 surviving individuals grown from each family. A minimum of 200 families were scored for the seedling forward-mutation frequency, and all selfed ears were scored for the seed defects. Mutations were scored as independent if they were not segregating in multiple families from the same founder. Phenotypic descriptions are available at [31], and it is expected that the grids not yet analyzed (ND) and the summer 2003 grids V, W, and X will be scored during 2004 and 2005 for reporting through the project database. Most mutations are caused by standard Mu elements.