Figure 5From: Development of a method for screening short-lived proteins using green fluorescent proteinCycloheximide chase analysis by western blot of three full-length myc-tagged cDNAs. Cells were transiently transfected to express splicing factor SRp30c, guanine nucleotide-binding regulatory protein or cervical cancer proto-oncogene p40, each with an amino-terminal myc epitope tag. Transfected cells were treated with CHX and samples subjected to western blot analysis using anti-myc antibody. The mobility of protein markers is indicated.Back to article page