Figure 4
From: Development of a method for screening short-lived proteins using green fluorescent protein
CHX chase analysis by western blot of three labile EGFP-fused library clones. (a) Cells were individually transfected with GFP-cDNA clones representing splicing factor SRp30c, guanine nucleotide-binding regulatory protein or cervical cancer proto-oncogene p40. Transfected cells were treated with CHX and were collected immediately thereafter or after 1, 2 or 3 hours for western blot analysis using anti-EGFP polyclonal antibody. The mobility of protein markers is indicated. (b) Cells were transfected with constructs expressing EGFP or d1EGFP, a destabilized form of GFP, and analyzed as in (a).