Figure 1
From: Development of a method for screening short-lived proteins using green fluorescent protein
Schematic diagram of the four steps of the screening procedure. (a) Fractionate by FACS cells transfected with an EGFP-cDNA expression library according to their fluorescence intensities; (b) refractionate those cells made dimmer by cycloheximide (CHX) treatment; (c) recover plasmids, clone in bacteria, pool clones and select CHX-responsive pools by FACS analysis; (d) recover and characterize individual cDNA clones.