Table 1 Overview of studies of gene-dosage effects in trisomies
From: Gene-dosage effects in Down syndrome and trisomic mouse models
| Â | Mao et al. [6] | Amano et al. [7] | Kahlem et al. [8] | Lyle et al. [9] |
|---|---|---|---|---|
Species and strain | Humans with Down syndrome | Ts1Cje mice | Ts65Dn mice | Ts65Dn mice |
Number of individuals | 4 plus 4 controls | 6 plus 6 controls | 4 plus 4 controls | 4 plus 4 controls |
Ages | 17-20 weeks gestation | Postnatal day 0 | 3-4 months | Postnatal day 30 and 11 months |
Tissues | Cerebrum and cortex-derived astrocyte cell lines | Whole brains | Cortex, midbrain, cerebellum, heart, testis, liver, kidney, lung, and muscle | Brain, liver, kidney, heart, muscle, and lung |
Technique used | Affymetrix microarrays | Affymetrix microarrays | Custom cDNA array | RT-PCR |
Number of genes on trisomic segment | Over 300 | 71 | 94 (77 tested in 9 tissues) | 94 (78 tested in 10 tissues) |
Trisomic genes increased in expression | 25 (variation among individuals) | 37 (variation among individuals) | All genes (of 66 with detectable expression) except in muscle and 10 gene-tissue combinations | 607 gene-tissue combinations (of 666 with detectable expression) |
Non-trisomic genes with altered expression | Approximately 185 | 258 | Not determined | Not determined |