Figure 1From: Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cellsIdentified proteins derived from the microsomes of AD and AS cells ordered by ratios of abundance. (a) Plot of the natural log d8/d0 ratio of the total number of ICAT reagent-labeled proteins derived from microsomes of 72 h AD (d0, light ICAT isotope reagent) and AS (10 nM R1881(methyltrienolone)) (d8, heavy ICAT isotope reagent) cells. Red and green represent androgen-induced and repressed protein abundance changes. A total of 1,064 proteins were identified and quantified (detailed in Materials and methods). (A list of all proteins is contained in panel 1 (pages 1-58) in Additional data file 1.) (b) PSA polypeptide sequence. Bold italics denote tryptic, cysteine-containing peptides. (c) Observed ICAT reagent-labeled PSA peptides. The number after each peptide sequence denotes the number of times that a peptide was identified. (d) PSA expression in the microsomes of AS cells. (e-l) Comparative immunoblot analysis of AD (d0) and AS (d8) microsomal extracts probed with antibodies to PSA, TMPRS22, FAS, LDLR, β-catenin, HSP60, MST-3 and FasL, respectively. Mass spectrometric d0/d8 ratios as displayed by the XPRESS quantification software [16] are shown below each blot. The asterisk denotes the cleaved processed form of the TMPRS22 protease [19].Back to article page