An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome

Table 2 Summary for the Heidelberg Collection R1

	Total	Heidelberg Predictions	BDGP Release 2/ BDGC R1	Other	Common predictions
Heidelberg Collection R1	21,396	7,464	483	71	13,378
Heidelberg PrimerSet	21,306	7,463	442	65	13,336
Heidelberg FlyArray	20,948	7,319	425	62	13,142
Expressed during development	13,927 (66.5%)	3,497 (47.8%)	232 (54.6%)	52 (83.9%)	10,146 (77.2%)
Validation by RT-PCR	386/478 (80.8%)	334/424 (78.8%)	ND	ND	52/54 (96.3%)

The Heidelberg Collection R1 resulted from the combination of our novel annotation with the published BDGP Release 2 annotation and the sequences of the BDGC R1 clones. The PrimerSet includes only those annotations for which we could successfully design primer pairs, and likewise, the Heidelberg FlyArray sums up the annotations that are included on our novel microarray. The next row presents the results of the developmental profiling; numbers given in parentheses are the percentage of annotations represented on the array that scored positive. The last row shows the validation rate of the microarray results by RT-PCR (amplicon length ≤750 bp).

ISSN: 1474-760X