| |
Total hits
|
Total conserved hits
|
1 site
|
≥ 2 sites
|
|---|
|
73 D. melanogaster miRNAs (A)
|
6,864
|
589
|
556
|
33
|
|
73 Random miRNAs (B)
|
5,152
|
204
|
201
|
3
|
|
Standard deviation (100 experiments)
|
± 132
|
± 43
|
± 40
|
± 3
|
|
Ratio (A/B)
| |
2.9
|
2.8
|
11.0
|
|
Estimated false positives (%)
| |
35%
|
36%
|
9%
|
- Detected conserved hits (especially those with multiple detected sites in the 3' UTR) are significantly over-represented (2.8× and 11× as many cases, on average, respectively) in analyses with actual miRNAs compared to randomly shuffled miRNAs. The thresholds used for this analysis were S: 100; ΔG: -19 kcal/mol; ID: 70%.
