Sequence comparison of the GAL1-GAL10 intergenic region across four yeast species. Scer, S. cerevisiae; Spar, S. paradoxus; Smik, S. mikatae; Sbay, S. bayanus. Arrows indicate the start and transcriptional orientation of the GAL1 and GAL10 open reading frames; dashes in the alignment indicate gaps; nucleotide positions conserved across all four species are denoted by asterisks. Stretches of conserved nucleotides are underlined, and experimentally validated transcription-factor binding-site footprints are boxed and labeled with the name of the footprinted transcription factor. Underlined regions that are not boxed correspond to potential, previously unknown, transcription-factor binding sites. Note that not all nucleotide positions of a footprinted binding site are necessarily conserved across all four species in this comparison (note the Mig1 sites, for example). The nucleotides matching the published Gal4 binding-site motif are in gray; for the fourth Gal4 site, non-standard consensus motif nucleotides are shown in boldface. Reproduced with permission from .