Multiple alignment of channel sequence from Anopheles gambiae and other organisms. (a) Voltage-gated K+-channel sequences; (b) Slo and Eag family K+ channels; (c) inward rectifier K+ channels. Alignments were generated with the ClustalX program, and highlighted with BOXSHADE. Sequences were chosen to illustrate diversity of Drosophila and mammalian K+-channel types, in addition to the Anopheles sequences. Transmembrane domains are labeled with a horizontal bar and the conserved GYG of the pore is marked by asterisks. The letter X represents regions at which amino-acid prediction was particularly difficult in Anopheles sequences, generally because of short exons in coding regions. Anopheles sequences are predictions based on regions homologous to Drosophila, as opposed to confirmed sequence data.