CpG content affects gene silencing in mice: evidence from novel transgenes

Table 1 Summary of LacZ expression pattern in transgenic animals

		YAC EF1αLacZ					EF1αLacZ				EF1αLagZ				EF1αLagoZ
Line (number of copies)		1 (1)	2 (1)	3 (1)	4 (2)	5 (1)	1 (N)	2 (6)	3 (N)	4 (N)	1 (>6)	2 (2)	3 (5)	4 (N)	1 (2)	2 (10-20)	3 (N)	4 (10-20)
Germ cells (pachytene)	Male	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
	Female	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
Mid gestation embryo (E9.5)		-	-	-	-	-	-	PD	PD	-	+	+	PD	PD	+	+	+	+
P7	CNS	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	+
	Epidermis	-	-	-	-	-	-	-	-	-	+	+	-	-	+	+	+	+
	Heart auricle	-	-	-	-	-	-	-	-	-	+	+	-	-	+	+	+	+
	Heart	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	+
	Endothelial cells	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Liver	-	-	-	-	-	-	-	-	-	+	+	-	-	+	+	+	+
	Lung	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Spleen	-	-	-	-	-	-	-	-	-	+	+	-	-	+	+	+	+
	Thymus	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Skeletal muscle	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Tendon cells	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
Adults	CNS	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	+
	Epidermis	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Heart auricle	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Heart	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-
	Endothelial cells	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Liver	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Lung	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-
	Spleen	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+
	Skeletal muscle	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
	Tendon cells	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	-	+

+, β-galactosidase positive; -, β-galactosidase negative; N, number of copies >20; PD, position (integration site) dependent pattern of expression. Expression pattern is variegated in EF1αLagZ2 line.

ISSN: 1474-760X