Figure 3
From: CpG content affects gene silencing in mice: evidence from novel transgenes
Methylation of reporter sequences of the genomic DNA extracted from tissue of animals hemizygous for the transgene. DNA was extracted from (a) liver and (b) testis. (c) DNA was digested with the enzyme indicated under the blots: EcoRI (E) for YacEF1αLacZ and EF1αLacZ liberating a 3.1-kb-long fragment; EcoRI and BamHI (B) for EF1αLagZ liberating a 3.4-kb-long fragment and EF1αLagoZ liberating a 3.5-kb-long fragment. Each vertical bar corresponds to an HpaII/MspI site. Probes are the complete reporter gene (Pr). For EF1αLagZ the EcoRI-BamHI-MspI digestion liberates one rearranged fragment indicated by a purple asterisk. The reference of the transgenic line is indicated at the top.