Figure 3
From: Characterizing the stress/defense transcriptome of Arabidopsis
Recovery of differentially expressed genes as a function of the number of clones screened. To reduce the redundant sequencing of clones, we pooled DNA from previously sequenced clones and used it as probes on new filters prepared from the stress libraries. As more clones were screened within a library, the fraction of genes that had not yet been recovered decreased.