Figure 2
From: Characterizing the stress/defense transcriptome of Arabidopsis
Differential screening of clones from the stress libraries generated using SSH. Subtracted cDNA fragments obtained by the SSH procedure were cloned (see Materials and methods for details) and maintained as bacterial cultures in 96-well plates for each library. Quadruplicate colony dot blots were prepared and the membranes hybridized with labeled unsubtracted cDNA probes derived from (a) the driver, (b) the unsubtracted cDNA probes from the tester, (c) the forward subtracted cDNAs or (d) the reverse-subtracted cDNA.