Figure 1
Multiple alignment of the conserved core of the rhomboid family proteins. The alignment includes the majority of the detected rhomboid family proteins; some closely related sequences were omitted. Only the six conserved (predicted) transmembrane helices (TMH) and short surrounding regions are shown. The boundaries of the predicted TMH are indicated by gray shading and overline and they are numbered 1-6. The number of amino-acid residues in the omitted terminal and internal regions are indicated. The consensus shows amino-acid residues present in at least 90% of the aligned sequences; h stands for hydrophobic residues (A, C, I, L, V, M, F, Y, W in the single-letter amino-acid code) and s for small residues (G, A, S, D, N, V). The proposed catalytic serine (TMH4) and histidine (TMH6) as well as conserved residues in TMH2 with possible ancillary roles in catalysis are highlighted in color. The proteins are identified with the gene identification (GI) number from the nonredundant database and an abbreviated species name. Bacterial species are color-coded green, eukaryotic species blue and archaeal species yellow. Species name abbreviations: Aerpe, Aeropyrum pernix; Agrtu, Agrobacterium tumefaciens; Anoga, Anopheles gambiae; Arath, Arabidopsis thaliana; Arcfu, Archaeoglobus fulgidus; Bacsu, Bacillus subtilis; Brume, Brucella melitensis; Caeel, Caenorhabditis elegans; Caucr, Caulobacter crescentus; Chlte, Chlorobium tepidum; Cloac, Clostridium acetobutilicum; Corgl, Corynebacterium glutamicum; Deira, Deinococcus radiodurans; Dicdi, Dictyostelium discoideum; Drome, Drosophila melanogaster; Escco, Escherichia coli; Haein, Haemophilus influenzae; Halsp, Halobacterium sp.; Homsa, Homo sapiens; Lacla, Lactococcus lactis; Lisin, Listeria innocua; Metja, Methanoccocus jannaschii; Metka, Methanopyrus kandleri; Metma, Methanosarcina mazei; Meslo, Mesorhizobium loti; Mycle, Mycobacterium leprae; Myctu, Mycobacterium tuberculosis; Neucr, Neurospora crassa; Nossp, Nostoc sp.; Prost, Providencia stuartii; Pyrab, Pyrococcus abyssi; Pyrae, Pyrobaculum aerophilum; Ralso, Ralstonia solanaraceum; Sacce, Saccharomyces cerevisiae; Schpo, Schizosaccharomyces pombe; Sinme, Sinorhizobium meliloti; Strco, Streptomyces coelicolor; Strpn, Streptococcus pneumoniae; Sulso, Sulfolobus solfataricus; Sulto, Sulfolobus tokodaii; Synsp, Synechocystis sp.; Theac, Thermoplasma acidophilum; Thema, Thermotoga maritima; Thete, Thermus thermophilus; Vibch, Vibrio cholerae; Xanca, Xanthomonas campestris; Xylfa, Xylella fastidiosa.