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Table 2 Summary of all libraries used for statistical analysis and correlation coefficients for comparisons made between SAGE libraries

From: MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues

Library name

Source

Tag number

ONL1

Adult mouse retina outer nuclear layer

26,016

ONL2

Same starting library as ONL1, but represents the second 26,000 tags sequenced in that library

25,956

P2.5A

P2.5 C57B/6 whole-mouse retina

23,525

P2.5B

Same ditag ligation as P2.5A was used to make the library, but ditag ligation stored at -20°C for 3 months before generation of library

23,566

Crx+/+A

P10.5 whole retina from Crx+/+ mice (hybrid of C57B/6 and Sv129)

17,441

Crx+/+B

Same mRNA sample as in Crx+/+A was used to make the library

17,422

41-year-old peripheral retina

41-year-old Japanese male

23,662

44-year-old peripheral retina

44-year-old American caucasian male

23,758

88-year-old peripheral retina

88-year-old American caucasian female

23,773

P6.5A

P6.5 whole retina from three different C57B/6 littermate mice. Tissue stored at -80°C for 1 week before library preparation

25,466

P6.5B

P6.5 whole retina from three different C57B/6 littermate mice, from same litter as for P6.5A. Tissue stored at -80°C for 6 months before library preparation

25,473

Hypothalamus A

8-week-old male C57B/6 mouse hypothalamus from three different individuals. Animals housed at facilities in Boston, MA

48,978

Hypothalamus B

8-week-old male C57B/6 mouse hypothalamus from 20 different individuals. Animals housed at facilities in Melbourne, Australia

48,953

Adult whole retina A

8-week-old male total C57B/6 mouse retina

48,964

Adult whole retina B

8-week-old male total C57B/6 mouse retina (same library as adult total retina A)

28,593

3T3 cells

Mouse 3T3 fibroblast cell line

28,531