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Table 2 Summary of all libraries used for statistical analysis and correlation coefficients for comparisons made between SAGE libraries

From: MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues

Library name Source Tag number
ONL1 Adult mouse retina outer nuclear layer 26,016
ONL2 Same starting library as ONL1, but represents the second 26,000 tags sequenced in that library 25,956
P2.5A P2.5 C57B/6 whole-mouse retina 23,525
P2.5B Same ditag ligation as P2.5A was used to make the library, but ditag ligation stored at -20°C for 3 months before generation of library 23,566
Crx+/+A P10.5 whole retina from Crx+/+ mice (hybrid of C57B/6 and Sv129) 17,441
Crx+/+B Same mRNA sample as in Crx+/+A was used to make the library 17,422
41-year-old peripheral retina 41-year-old Japanese male 23,662
44-year-old peripheral retina 44-year-old American caucasian male 23,758
88-year-old peripheral retina 88-year-old American caucasian female 23,773
P6.5A P6.5 whole retina from three different C57B/6 littermate mice. Tissue stored at -80°C for 1 week before library preparation 25,466
P6.5B P6.5 whole retina from three different C57B/6 littermate mice, from same litter as for P6.5A. Tissue stored at -80°C for 6 months before library preparation 25,473
Hypothalamus A 8-week-old male C57B/6 mouse hypothalamus from three different individuals. Animals housed at facilities in Boston, MA 48,978
Hypothalamus B 8-week-old male C57B/6 mouse hypothalamus from 20 different individuals. Animals housed at facilities in Melbourne, Australia 48,953
Adult whole retina A 8-week-old male total C57B/6 mouse retina 48,964
Adult whole retina B 8-week-old male total C57B/6 mouse retina (same library as adult total retina A) 28,593
3T3 cells Mouse 3T3 fibroblast cell line 28,531