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Table 1 Fully sequenced cycad clones from contigs that match only genes in gymnosperms

From: Expressed sequence tag analysis in Cycas, the most primitive living seed plant

Contig GenBank accession number Transcript length (bp) Peptide (residues) InterPro result
gy79c04_704 CB090702 586 72 No matches found
gy78g12_244 CB090673 627 84 No matches found
te82h02_741 CB093328 647 107 No matches found
he95e08_721 CB091708 651 114 No matches found
hf04g07_288 CB092366 684 141 ASP_RICH (unintegrated)
hk42a07_743 CB093061 790 142 No matches found
gp23c01_369 CB089407 791 189 No matches found
gp26f08_297 CB089628 827 69 No matches found
gy82g05_181 CB090964 840 118 No matches found
he92f06_688 CB091462 935 170 No matches found
gy81e11_544 CB090877 948 211 ASP_RICH (unintegrated)
he97c12_740 CB091858 965 140 No matches found
gp32b03_724 CB089926 1311 335 Peptidoglycan-binding LysM
te83a03_729 CB093338 1899 527 No matches found
Average   893 173  
  1. All available ESTs and annotated genes from GenBank were divided into three categories (angiosperms, gymnosperms and lower plants) and compared with the C. rumphii UniGene set. Forty-six cycad ESTs that had no similarity to angiosperm genes, but matched gymnosperm and lower plant genes, were fully sequenced, of which 14 clones (listed) still have no similarity to angiosperms. To confirm that these genes were of cycad origin, all 14 were successfully amplified from the DNA of a C. rumphii specimen other than the one used to construct the cDNA library. The transcript length, as well as the predicted translation product size, is shown. Interpro analysis identified conserved motifs in three of these cycad ESTs as shown.