Expressed sequence tag analysis in Cycas, the most primitive living seed plant

Table 1 Fully sequenced cycad clones from contigs that match only genes in gymnosperms

Contig	GenBank accession number	Transcript length (bp)	Peptide (residues)	InterPro result
gy79c04_704	CB090702	586	72	No matches found
gy78g12_244	CB090673	627	84	No matches found
te82h02_741	CB093328	647	107	No matches found
he95e08_721	CB091708	651	114	No matches found
hf04g07_288	CB092366	684	141	ASP_RICH (unintegrated)
hk42a07_743	CB093061	790	142	No matches found
gp23c01_369	CB089407	791	189	No matches found
gp26f08_297	CB089628	827	69	No matches found
gy82g05_181	CB090964	840	118	No matches found
he92f06_688	CB091462	935	170	No matches found
gy81e11_544	CB090877	948	211	ASP_RICH (unintegrated)
he97c12_740	CB091858	965	140	No matches found
gp32b03_724	CB089926	1311	335	Peptidoglycan-binding LysM
te83a03_729	CB093338	1899	527	No matches found
Average		893	173

All available ESTs and annotated genes from GenBank were divided into three categories (angiosperms, gymnosperms and lower plants) and compared with the C. rumphii UniGene set. Forty-six cycad ESTs that had no similarity to angiosperm genes, but matched gymnosperm and lower plant genes, were fully sequenced, of which 14 clones (listed) still have no similarity to angiosperms. To confirm that these genes were of cycad origin, all 14 were successfully amplified from the DNA of a C. rumphii specimen other than the one used to construct the cDNA library. The transcript length, as well as the predicted translation product size, is shown. Interpro analysis identified conserved motifs in three of these cycad ESTs as shown.

ISSN: 1474-760X