Figure 4

Slot-blot analysis, library screening and Southern blot of ascidian non-LTR elements. (a) On the left is shown a representative experiment of slot-blot analysis of CiLOA elements in three specimens with (from left to right) 500 ng, 250 ng, 50 ng and 25 ng of EcoRI-digested C. intestinalis genomic DNA. On the right is shown slot-blot analysis of serial dilutions of plasmid containing CiLOA which has been EcoRI-restricted and mixed with 1 μg mouse DNA. (b) Hybridization of a C. intestinalis genomic library screened with CiLOA. Positive signals have been depicted (from a to v) in the original (left) and its duplicate (right). (c) The first four panels show Southern analyses of 10 μg C. intestinalis genomic DNA digested with HpaII (H) or MspI (M) and probed with the non-LTR element indicated under each panel; the fifth panel shows 10 μg Branchiostoma floridae (amphioxus) genomic DNA digested with HpaII (H), MboI (Mb), MspI (M) or Sau3A (S) and probed with BfCR1.