Odorant receptor expressed sequence tags demonstrate olfactory expression of over 400 genes, extensive alternate splicing and unequal expression levels

Table 2 Summary of cDNA screen for each library and probe.

Library	Probe	Number of plaques screened (× 10³)	Number of sequences obtained	Number of real olfactory receptor sequences	True-positive rate	Olfactory receptor clone frequency	Number of olfactory receptor genes represented
Embryonic	OR5B_OR3B_40	640	58	37	64%	1/17,300	27
Adult	OR5B_OR3B_40	2,850	1,450	1,138	78%	1/2,500	394
	P24_P28_40 and TM3deg1_P28_45	200	23	3	13%	1/66,700	3
	P26_P27_45	700	135	58	43%	1/12,100	35
	P24_P28_45	200	39	22	56%	1/9,100	19
	OR5B_OR3B_45	150	10	6	60%	1/250,000	5
Total		4,740	1,715	1,264	74%	1/3,800	419

Probe names comprise the names of the two primers and the annealing temperature used during PCR to generate the probes, separated by underscores

ISSN: 1474-760X