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Table 2 Summary of cDNA screen for each library and probe.

From: Odorant receptor expressed sequence tags demonstrate olfactory expression of over 400 genes, extensive alternate splicing and unequal expression levels

Library Probe Number of plaques screened (× 103) Number of sequences obtained Number of real olfactory receptor sequences True-positive rate Olfactory receptor clone frequency Number of olfactory receptor genes represented
Embryonic OR5B_OR3B_40 640 58 37 64% 1/17,300 27
Adult OR5B_OR3B_40 2,850 1,450 1,138 78% 1/2,500 394
  P24_P28_40 and TM3deg1_P28_45 200 23 3 13% 1/66,700 3
  P26_P27_45 700 135 58 43% 1/12,100 35
  P24_P28_45 200 39 22 56% 1/9,100 19
  OR5B_OR3B_45 150 10 6 60% 1/250,000 5
Total   4,740 1,715 1,264 74% 1/3,800 419
  1. Probe names comprise the names of the two primers and the annealing temperature used during PCR to generate the probes, separated by underscores