Figure 8

Sensitivity of junction probes to small sequence variations. Tiling probes were stepped across RB1 exon-exon junctions incorporating artificial (a) insertions or (b) deletions simulating mRNA nucleotide deletions and insertions, respectively. Nucleotides were deleted separately from each exon of a junction, resulting in two intensity profiles for each deletion size, averaged together in the lower panel. Data are from 40-mers spanning exon 9 to exon 10 pseudojunctions, hybridized with Jurkat mRNA in 35% formamide.