Figure 3

Probe-intensity profiles for intron-exon boundaries (edges) of the 27 RB1 exons using 35-nucleotide probes and 30% formamide, where each color represents a single exon. (a) 3' splice sites (5' exon edge); (b) 5' splice sites (3' exon edge). These conditions allow accurate prediction of splice sites. The dashed line marks actual splice sites, by which the exons were aligned, and the small circles mark the predicted splice-site locations for each exon after convolution with the Haar wavelet (see Materials and methods for details). Although the short exon-10 sequence contains several AT-rich regions within the exon which hybridize poorly and create a dip in the intensity profile (lower magenta curve), the splice-site predictions for exon 10 are consistent with the others.