Figure 1From: Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicingFlow chart of the full-length amplification and labeling protocol. The TotRP-Amp protocol (upper left) starts with total RNA whereas mRP-Amp starts with mRNA. Both methods result in labeled complementary cDNA (ccDNA) sufficient for six hybridizations. dsDNA, double-stranded DNA. See Materials and methods for details.Back to article page