Figure 1

Schematic representation of the EST analysis and annotation process. ESTs from the 5' end of cDNAs were repeat masked and checked for sequence integrity. They were BLAST-searched against the NCBI nr database, the mouse dbEST and our local database of EST sets from each library (individual library ESTs) or the entire collection of 9,638 ESTs (all three library ESTs). High-level functional annotation was achieved by searching the NCBI databases, including Entrez nt, LocusLink, mouse and human UniGene database. Databases that were BLASTed or queried are indicated by solid blue, rounded rectangles. Annotated data (highlighted by dashed, yellow rectangles) for every EST can be accessed at [31].