Figure 2
From: Tackling an essential problem in functional proteomics of Saccharomyces cerevisiae
Schematic outline of the procedure for td-gene inactivation. Horizontal arrows indicate the anticipated results at each step of the process. Procedures in parentheses are optional steps that may be used to characterize the td protein's function within the cell cycle. Alternative treatments may be used before and/or after td-protein inactivation to determine its effect on different processes. See text for additional description of the scheme.