Figure 9

Specificity of 70-mer oligonucleotide probes. An oligonucleotide probe (70-mer) was designed to detect Cy-labeled H⁺-ATPase cDNA (target) reverse transcribed from rat heart and brain RNA. Nucleotides 1-70 of the oligonucleotide probe correspond to nucleotides 72-141 of an EST clone for H⁺-ATPase (GenBank accession BM986597). This EST clone serves as the DNA template for generating the PCR amplicon probe. For orientation, the 70-mer oligonucleotide sequence is shown aligned with nucleotides 669-738 of the full-length H⁺-ATPase mRNA (GenBank accession D10874). Contained within the 70-mer oligonucleotide probe is a stretch of 20 contiguous nucleotides that is also found in the tumor endothelial marker 8 mRNA (GenBank accession AF378762). Other than this stretch of contiguous nucleotides, there is no sequence identity between H⁺-ATPase and tumor endothelial marker 8.