Figure 7From: Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarraysThe role of oligonucleotide concentration on microarray hybridization measurements. (a) Comparison of hybridization intensities of seven genes detected by oligonucleotides printed at different concentrations. Corresponding PCR amplicons at 100-200 ng/μl are included for comparison. The medium background subtracted fluorescent intensities were summed for the Cy3 and Cy5 channels for each gene and plotted. (b) Comparison of the expression ratios of differentially expressed genes detected by oligonucleotides printed at different concentrations. Data are the mean ± SD of four independent experiments.Back to article page