Figure 1

Retention of unmodified rat 70-mer oligonucleotide and cDNA probes printed on the same array. 70-mer oligonucleotides (50 μM) and PCR amplicons (100-200 nM; insert size ranged from 0.5 to 1.5 kb) were printed onto TeleChem SuperAmine slides and immobilized by UV cross-linking at 250 mJ/cm². The image depicts DNA probes deposited onto slides stained with Vistra Green nucleic acid staining solution. (a) Section of array after staining. (b) The same section of array after the slide was extensively washed overnight in 0.2% SDS at room temperature, restained and scanned. (c) Average DNA fluorescence intensity of target hybridization to PCR amplicon and oligonucleotide probes before and after the extensive wash. Signal intensities were obtained from GenePix 3.0 software (Axon Instruments, CA) after scanning at 532 nm. Results are representative of 10 independent experiments.