Figure 2

Schematic representation of time-zero transformation. In this process, the logR of the zero time point is subtracted from each time point in the time course. Zero transformation enables visual comparison of trends in gene expression for different genes in the same time course, and also for trends in gene expression from different experiments. (a) Time course of three different genes, plotted as the logR relative to the reference pool of mRNA. (b) Zero transformation of the time courses in (a). (c) Visual representation of trends in gene expression for untransformed data (a) as seen in TreeView. The Color scheme depicts LogR values with gray equalling zero, red > 0, and green < 0. (d) TreeView image of zero-transformed data.